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ptriex rhoa fret wt biosensor  (Addgene inc)


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    Addgene inc ptriex rhoa fret wt biosensor
    Ptriex Rhoa Fret Wt Biosensor, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ptriex+rhoa+fret+wt+biosensor/pmc12307676-28-0-5?v=Addgene+inc
    Average 92 stars, based on 29 article reviews
    ptriex rhoa fret wt biosensor - by Bioz Stars, 2026-07
    92/100 stars

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    The Decrease in Membrane Tension during Early Differentiation Is Induced by a β-Catenin and <t>RhoA-Mediated</t> Decrease in ERM Phosphorylation (A) Fluorescent Western blot and associated quantification for pERM and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) of WT and β-catenin knockout (KO) cells cultured in 2i+L (N = 6). (B) Trap force measurements of β-catenin KO ESCs and WT ESCs and T24 spread (S) cells (N = 3). (C) Schematic of the <t>FRET</t> sensor for RhoA activity. RBD, Rho binding domain. (D) Representative images of the bright-field and FRET ratio of WT ESCs, T24 cells, and β-catenin KO ESCs expressing the RhoA activation FRET sensor. (E) Quantification of the average FRET ratio (~RhoA activity) per cell (N = 3). (F) Active RhoA pull-down assay. Top, representative fluorescent Western blot for RhoA in WT ESCs, T24, and β-catenin KO cells after active RhoA pull-down. Bottom, quantification of active RhoA pulled down. (N = 3). (G) Top, representative fluorescent Western blot for pERM and GAPDH in WT ESCs, iRhoA_CA ESCs, and T24 cells. Note that WT 2i+L is the same as in (A) (A and G are on the same gel). Bottom, corresponding quantification (N = 4). (H) Trap force measurement of WT ESCs, T24S, iRhoA_CA ESCs, and T24 cells (N = 3). WT data in (B) and (H) are from <xref ref-type=Figure 1 E. The graphical data represent means ± SDs. p values established by Welch’s unpaired Student’s t test and indicated in the figure. Scale bars represent 10 μm. " width="250" height="auto" />
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    The Decrease in Membrane Tension during Early Differentiation Is Induced by a β-Catenin and RhoA-Mediated Decrease in ERM Phosphorylation (A) Fluorescent Western blot and associated quantification for pERM and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) of WT and β-catenin knockout (KO) cells cultured in 2i+L (N = 6). (B) Trap force measurements of β-catenin KO ESCs and WT ESCs and T24 spread (S) cells (N = 3). (C) Schematic of the FRET sensor for RhoA activity. RBD, Rho binding domain. (D) Representative images of the bright-field and FRET ratio of WT ESCs, T24 cells, and β-catenin KO ESCs expressing the RhoA activation FRET sensor. (E) Quantification of the average FRET ratio (~RhoA activity) per cell (N = 3). (F) Active RhoA pull-down assay. Top, representative fluorescent Western blot for RhoA in WT ESCs, T24, and β-catenin KO cells after active RhoA pull-down. Bottom, quantification of active RhoA pulled down. (N = 3). (G) Top, representative fluorescent Western blot for pERM and GAPDH in WT ESCs, iRhoA_CA ESCs, and T24 cells. Note that WT 2i+L is the same as in (A) (A and G are on the same gel). Bottom, corresponding quantification (N = 4). (H) Trap force measurement of WT ESCs, T24S, iRhoA_CA ESCs, and T24 cells (N = 3). WT data in (B) and (H) are from <xref ref-type=Figure 1 E. The graphical data represent means ± SDs. p values established by Welch’s unpaired Student’s t test and indicated in the figure. Scale bars represent 10 μm. " width="100%" height="100%">

    Journal: Cell Stem Cell

    Article Title: Membrane Tension Gates ERK-Mediated Regulation of Pluripotent Cell Fate

    doi: 10.1016/j.stem.2020.10.018

    Figure Lengend Snippet: The Decrease in Membrane Tension during Early Differentiation Is Induced by a β-Catenin and RhoA-Mediated Decrease in ERM Phosphorylation (A) Fluorescent Western blot and associated quantification for pERM and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) of WT and β-catenin knockout (KO) cells cultured in 2i+L (N = 6). (B) Trap force measurements of β-catenin KO ESCs and WT ESCs and T24 spread (S) cells (N = 3). (C) Schematic of the FRET sensor for RhoA activity. RBD, Rho binding domain. (D) Representative images of the bright-field and FRET ratio of WT ESCs, T24 cells, and β-catenin KO ESCs expressing the RhoA activation FRET sensor. (E) Quantification of the average FRET ratio (~RhoA activity) per cell (N = 3). (F) Active RhoA pull-down assay. Top, representative fluorescent Western blot for RhoA in WT ESCs, T24, and β-catenin KO cells after active RhoA pull-down. Bottom, quantification of active RhoA pulled down. (N = 3). (G) Top, representative fluorescent Western blot for pERM and GAPDH in WT ESCs, iRhoA_CA ESCs, and T24 cells. Note that WT 2i+L is the same as in (A) (A and G are on the same gel). Bottom, corresponding quantification (N = 4). (H) Trap force measurement of WT ESCs, T24S, iRhoA_CA ESCs, and T24 cells (N = 3). WT data in (B) and (H) are from Figure 1 E. The graphical data represent means ± SDs. p values established by Welch’s unpaired Student’s t test and indicated in the figure. Scale bars represent 10 μm.

    Article Snippet: Rab5a and FRET plasmids were provided by Giorgio Scita’s lab ( ) Ezr_GFP and EZR_CA_GFP were provided by Guillaume Charras ( ) FRET RhoA sensor was obtained from Adgene (#12150) and is characterized in )

    Techniques: Membrane, Phospho-proteomics, Western Blot, Knock-Out, Cell Culture, Activity Assay, Binding Assay, Expressing, Activation Assay, Pull Down Assay